Sequences from confirmation amplicon


Confirmation sequences for line 083D05 and hit At1g75000 are shown.
Line ID 083D05
Locus At1g75000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28164627
Primers used for PCR 04us - 8474, show primer details
   
Sequence Name 88-K118629-0022-083-D05C-04us
GenBank Accession LN514901 [GenBank]
Sequencing primer gene specific primer, 04us: 5'-TTCAATACCGAAAGCTACCTGACT-3'
Sequence (A. th genome BLAST matches underlined) 
>88-K118629-0022-083-D05C-04us
AATCTTCAAAGATCAAATAAGCTATTATAATGCAAAATTATATAATATATTTTGTGTCGG
AGAGCAAGACTACAATGATTCCTATTCCTAATTATCATACAACAACAACATCAACAGAAT
AAATCTGATCTCCAGTTTTCATATTTTAGCTGAAGTCTGATTTTTAACAATAAATCAATA
TGGCTATATTCAACATTCATGTCCTATAATTGTTGTATAATAAATCATGTTATATTTTTA
GCTATTCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCGCAGCGTTATTATACTNANNTTNTGGGACATGAATGTTGAA
TATAGCCATATTGATTTATTGTTAAAAATCAGACTTCAGCTAAAATATGAAAACTGGAGA
TCAGATTTATTCTGTTGATGTTGTTGTTGTATGATAATTAGGAATAGGAATCATTGTAGT
CTTGCTCTCCGACACAAAATATATTATATAATTTTGCATTATAATAGCTTATTTTGATCT
TTTGAAGATTCGTCACTTTTCTCAGGTTGCAAGGAAAGAAAAAGTCAGGTAGCTTTCGG
   
Sequence Name 69-K118628-0022-083-D05C-8409
GenBank Accession LN512287 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K118628-0022-083-D05C-8409
AGCCATTTACATTGAATTATCCTGGAATAGCTAAAAATATAACATGATTTATTATACAAC
AATTATAGGACATGAATGTTGAATATAGCCATATTGATTTATTGTTAAAAATCAGACTTC
AGCTAAAATATGAAAACTGGAGATCAGATTTATTCTGTTGATGTTGTTGTTGTATGATAA
TTAGGAATAGGAATCATTGTAGTCTTGCTCTCCGACACAAAATATATTATATAATTTTGC
ATTATAATAGCTTATTTTGATCTTTTGAAGATTCGTCACTTTTCTCAGGTTGCAAGGAAA
GAAAAAGTCAGGTAGCTTTCGGTATTGAAATTCCTCGGTTGGGCTTGGGACAACAACCAC
CGCCTAAAATCACACGAAAACTAGCA

Last Updated on 06.12.2018 16:11