Genotyping and primer details


Genotyping and primer details for 083D05 - At1g75000
Line ID 083D05
Locus At1g75000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:28164627 of line 083D05 Chr1:28164627
   
Insertion allele amplicon 8474 - 04us
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 04us: 5'-TTCAATACCGAAAGCTACCTGACT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:28164849
   
wt amplicon (over InsPos) 43vn - 04us
wt amplicon start Chr1:28164118
wt amplicon end Chr1:28164849
wt amplicon size 732 bp
Locus specific primer 04us: 5'-TTCAATACCGAAAGCTACCTGACT-3'
2nd locus specific primer 43vn: 5'-TTCCAGCTTATCAACCAGTCTTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11