Sequences from confirmation amplicon


Confirmation sequences for line 086H03 and hit At5g17167 are shown.
Line ID 086H03
Locus At5g17167 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5645493
Primers used for PCR 15so - 3144, show primer details
   
Sequence Name 37-K116453-0022-086-H03C-15so
GenBank Accession LN502746 [GenBank]
Sequencing primer gene specific primer, 15so: 5'-AGCTTTAGGACAAGCTGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K116453-0022-086-H03C-15so
AGGACGAGCTGAGGAGCTTGAGGACGAGCTGATAAGCTTGAGGACGAGCAGAGGAGCTAG
AGAACGAGCTGAGGAGCTTGAAGACGTTTTAATATGGATTTCTTCCTCCTTTTTTCCTTG
AGATGCCTCTCTATTTATAGAAAAACTAAGCTGGTGATAGGATAATGGACCAACTTTTAT
GGGCTTTTTGGGCTTGATGTTGATTAGTTTATTCTTGATTTGGGCCGAGCTAAAATGAGG
GGTGCAATCCATCCCCCAACATGCCCCATGGAGCTTTCTGTATTTTTCTTCCCCAGAAGC
TCAAACATCAACAGTTATTCACTAAAAGGTACTTAAATACATGGCTTAATTCCTACAAAA
TTCTACTACTTGCAATTAAATTCATACTTTAAATATCTCAAATCGAAACTGTAACTGGAG
AAAAGCAAAAACCTAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCC
CCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCG
AAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCCAACCAAATCCACA
GGGTT
   
Sequence Name 04-K116452-0022-086-H03C-3144
GenBank Accession LN488069 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K116452-0022-086-H03C-3144
CTTCCTCTATATAAGGAAGTTCATTTAATTTGGAGAGGACAGGGTACCCGGGGATCAGAT
TGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTAGGTTTTTGCTTTTCTCCAGTTA
CAGTTTCGATTTGAGATATTTAAAGTATGAATTTAATTGCAAGTAGTAGAATTTTGTAGG
AATTAAGCCATGTATTTAAGTACCTTTTAGTGAATAACTGTTGATGTTTGAGCTTCTGGG
GAAGAAAAATACAGAAAGCTCCATGGGGCATGTTGGGGGATGGATTGCACCCCTCATTTT
AGCTCGGCCCAAATCAAGAATAAACTAATCAACATCAAGCCCAAAAAGCCCATAAAAGTT
GGTCCATTATCCTATCACCAGCTTAGTTTTTCTATAAATAGAGAGGCATCTCAAGGAAAA
AAGGAGGAAGAAATCCATATTAAAACGTCTTCAAGCTCCTCAGCTCGTTCTCTAGCTCCT
CTGCTCGTCCTCAAGCTTATCAGCTCGTCCTCAAGCTCCTCAGCTCGTCCTTAAGCTCAG
CTCGTTCTCAAGCTCCTCTGCTCGTCCTCAAGCTTTTCAGCTTGCCCTTAAAGCTAAGAT
AAATCGGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGAC
TCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAA
TGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATC

Last Updated on 06.12.2018 16:11