Sequences from confirmation amplicon


Confirmation sequences for line 097F09 and hit At3g17860 are shown.
Line ID 097F09
Locus At3g17860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6122269
Primers used for PCR 05vd - 3144, show primer details
   
Sequence Name 68-K117751-0022-097-F09C-05vd
GenBank Accession LN512088 [GenBank]
Sequencing primer gene specific primer, 05vd: 5'-ATTAGGTTGCAGAGCTGAGAGAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K117751-0022-097-F09C-05vd
AAGCAATATGGGGATACGCTCGTGACCCTGAGAAACCCACAGCGAGAAAGAGAGTTAGCC
CAAGAAACTATTGAATGGTGTATTCTAAAGAAGTGTTGCGTACCTTTCTTTGCGTTTCTC
TAAAAACCTAGCCAGGGATGCTTTGCGAGCCAGAGGTAATGCCACTGCATAAGAAACAGA
ACGAGAATGAAGTTACATCCATGAAAATACTAGTAAGTTAGATGGCGAATGTAGTATCAG
GTCTCTGACTTTGTTTTTTACCTGTTGGAGCCATTACATTGGTAGAACAAGAAACTGGTA
CTGGGCAGTTAATATTGGATCCATTAGCTTGGTTGTTGTGATATGTTTAAACCGAAGGCG
GGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTAT
ATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAG
ATA
   
Sequence Name 43-K117750-0022-097-F09C-35St
GenBank Accession LN504988 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K117750-0022-097-F09C-35St
TCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCG
TTTCCCGCCTTCGGTTTAAACATATCACAACAACCAAGCTAATGGATCCAATATTAACTG
CCCAGTACCAGTTTCTTGTTCTACCAATGTAATGGCTCCAACAGGTAAAAAACAAAGTCA
GAGACCTGATACTACATTCGCCATCTAACTTACTAGTATTTTCATGGATGTAACTTCATT
CTCGTTCTGTTTCTTATGCAGTGGCATTACCTCTGGCTCGCAAAGCATCCCTGGCTAGGT
TTTTAGAGAAACGCAAAGAAAGGTACGCAACACTTCTTTAGAATACACCATTCAATAGTT
TCTTGGGCTAACTCTCTTTCTCGCTGTGGGTTTCTCAGGGTCACGAGCGTATCCCCATAT
TGCTTAGACAAGAAGTCATCGACAGATTGTCGCAGATCAATGTCTGAATGCATTAGTTCT
TCTCTCAC

Last Updated on 06.12.2018 16:11