Genotyping and primer details


Genotyping and primer details for 097F09 - At3g17860
Line ID 097F09
Locus At3g17860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:6122269 of line 097F09 Chr3:6122269
   
Insertion allele amplicon 3144 - 05vd
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 05vd: 5'-ATTAGGTTGCAGAGCTGAGAGAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:6122692
   
wt amplicon (over InsPos) 62vo - 05vd
wt amplicon start Chr3:6122033
wt amplicon end Chr3:6122692
wt amplicon size 660 bp
Locus specific primer 05vd: 5'-ATTAGGTTGCAGAGCTGAGAGAAG-3'
2nd locus specific primer 62vo: 5'-CTTTCTTTCATTTCTCAGGCAAAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11