Sequences from confirmation amplicon


Confirmation sequences for line 098E12 and hit At4g24972 are shown.
Line ID 098E12
Locus At4g24972 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12839464
Primers used for PCR 05wu - 8474, show primer details
   
Sequence Name 87-K118926-0022-098-E12C-05wu
GenBank Accession LN514808 [GenBank]
Sequencing primer gene specific primer, 05wu: 5'-TGCTTAAACAGCTCCCTACTTCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>87-K118926-0022-098-E12C-05wu
ATTCTCTTTTTTCATTTTAACTCCAATATTTTTTTGGTTATTCCATTAAATTTTTACTAG
AAAATGTAGTTAGAGAGAACGAGTCTTTAGCATATCCTCTTAGCAATGTAGCTACTAATA
TACTGAAATTCCTCTGTTTTGATTGTGTGTGTGATTGTTTATATAAAGGAAAAACAGAAA
GGAGTGTAGAGCCGGAGAGAATTGGAGAAAAGTGCAAGAGTACGGACATAGTGGTGAACC
AAGCAGTAACGGAACCAATGCCTAACGGAATACCAGGCTACATGGTAGAGATAACGAACC
AGTGTATGTCTGGATGCATAATTTCGAGGATCCACATCAACTGCGGTTGGTTCAGCTCAG
GACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGACCGCACGGTTATT
   
Sequence Name 63-K118925-0022-098-E12C-8409
GenBank Accession LN511095 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K118925-0022-098-E12C-8409
ATTGAATATATCCTGTCCTGAGCTGAACAACCGCAGTTGATGTGGATCCTCGAAATTATG
CATCCAGACATACACTGGTTCGTTATCTCTACCATGTAGCCTGGTATTCCGTTAGGCATT
GGTTCCGTTACTGCTTGGTTCACCACTATGTCCGTACTCTTGCACTTTTCTCCAATTCTC
TCCGGCTCTACACTCCTTTCTGTTTTTCCTTTATATAAACAATCACACACACAATCAAAA
CAGAGGAATTTCAGTATATTAGTAGCTACATTGCTAAGAGGATATGCTAAAGACTCGTTC
TCTCTAACTACATTTTCTAGTAAAAATTTAATGGAATAACCAAAAAAATATTGGAGTATA
AAATGAAAAAAAGAGAATACTAGTATTAATTTGACAGCTGGATGAAGTAGGGAGTGNTTT
AAGC

Last Updated on 06.12.2018 16:11