Genotyping and primer details


Genotyping and primer details for 098E12 - At4g24972
Line ID 098E12
Locus At4g24972 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:12839464 of line 098E12 Chr4:12839464
   
Insertion allele amplicon 8474 - 05wu
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 05wu: 5'-TGCTTAAACAGCTCCCTACTTCAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:12839056
   
wt amplicon (over InsPos) 41xh - 05wu
wt amplicon start Chr4:12839056
wt amplicon end Chr4:12840198
wt amplicon size 1143 bp
Locus specific primer 05wu: 5'-TGCTTAAACAGCTCCCTACTTCAT-3'
2nd locus specific primer 41xh: 5'-CCAGTATATTATTCAAGCCAAGGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11