Sequences from confirmation amplicon


Confirmation sequences for line 100H05 and hit At3g57420 are shown.
Line ID 100H05
Locus At3g57420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21252666
Primers used for PCR BA49 - 3144, show primer details
   
Sequence Name 26-K105032-0022-100-H05C-BA49
GenBank Accession LN497920 [GenBank]
Sequencing primer gene specific primer, BA49: 5'-AATGTTTGGTTTGAGGAGTTATGG-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K105032-0022-100-H05C-BA49
TTTTTAGGTAACGGAGTCATGGACAACGGTGAGACCAGCCGGGAACTCGGATTGGTATTC
GGTTCAGGCGGAATTAGTCAAGAAAATTGTCAGTACAATGCCGGTTCATTTCCAAGTCAA
TTACAAGGAAGCCACAAAGAATAGCGATGGCACAAGCTTAACGATGTGCAGCTCGGAAGT
TTTCTATGTCCCTAAACGCTTCGTCTCAGACTTTACAGATCTTGTTAATCTCGTTGGTGA
TATGGATTTGCATTACAAAGTTGCTGTGCCTATGTTCTTCCTATCGATGGATTCGCCTCA
GAATTTTGACCCGGTTCTAGGCTCAATGGTATATAAGTCAGAACCAGCTTCGCTAAACTC
TTCTTTGAGTTTGTATTCAGCGGAAGCACCTGCAGTTCATCCTTGGAGCATCTCGAACGA
ACAAGACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCT
GTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGG
GATTGTGCGTCATCCCTTACGTCAGTGGAGATATC
   
Sequence Name 42-K105033-0022-100-H05C-3144
GenBank Accession LN504458 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K105033-0022-100-H05C-3144
TCCACTATCCTTGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACA
GGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTCTTGTT
CGTTCGAGATGCTCCAAGGATGAACTGCAGGTGCTTCCGCTGAATACAAACTCAAAGAAG
AGTTTAGCGAAGCTGGTTCTGACTTATATACCATTGAGCCTAGAACCGGGTCAAAATTCT
GAGGCGAATCCATCGATAGGAAGAACATAGGCACAGCAACTTTGTAATGCAAATCCATAT
CACCAACGAGATTAACAAGATCTGTAAAGTCTGAGACGAAGCGTTTAGGGACATAGAAAA
CTTCCGAGCTGCACATCGTTAAGCTTGTGCCATCGCTATTCTTTGTGGCTTCCTTGTAAT
TGACTTGGAAATGAACCGGCATTGTACTGACAATTTTCTTGACTAATTCCGCCTGAACCG
AATACCAATCCGAGTTCCCGGCTGGTCTCACCGTTGTCCATGACTCCGTTACCTAAAAAA
GGGACTCTTTGTCATAAAACCGAATCCAACCATAACTCCTCA

Last Updated on 06.12.2018 16:11