Genotyping and primer details


Genotyping and primer details for 100H05 - At3g57420
Line ID 100H05
Locus At3g57420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:21252666 of line 100H05 Chr3:21252666
   
Insertion allele amplicon 3144 - BA49
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer BA49: 5'-AATGTTTGGTTTGAGGAGTTATGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:21253145
   
wt amplicon (over InsPos) BA50 - BA49
wt amplicon start Chr3:21252084
wt amplicon end Chr3:21253145
wt amplicon size 1062 bp
Locus specific primer BA49: 5'-AATGTTTGGTTTGAGGAGTTATGG-3'
2nd locus specific primer BA50: 5'-ACTTTGAATATATGTTGCCGGTTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11