Sequences from confirmation amplicon


Confirmation sequences for line 114D10 and hit At4g39030 are shown.
Line ID 114D10
Locus At4g39030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:18188851
Primers used for PCR 04nt - 3144, show primer details
   
Sequence Name 68-K114626-0022-114-D10C-04nt
GenBank Accession LN512066 [GenBank]
Sequencing primer gene specific primer, 04nt: 5'-TTGATCTTTTATGCCAAATTCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K114626-0022-114-D10C-04nt
TTGATCTTTTCTAACTGATTGAACTAACCACACTTGTATATATTATTTGTCTATTATGCT
TTAAATTTACATTGAGCCTCTGACCAAAGAAAAAAGTTGACAATAATTAGCCGTTTCTTG
ACATTGGTGCCTGATATATCATAATTAGCCATTTGTTGGCAAACTCTTAGATAATCAATG
AGATCATCAAACTGAGACTTAAATGGATTTAATCTTCTCCACCGTGTATGGACTCGGCCC
ATCTGATAGTTTAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCC
GGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAA
GGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCAAAACAATCCA
   
Sequence Name 68-K114625-0022-114-D10C-3144
GenBank Accession LN512065 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K114625-0022-114-D10C-3144
CAGACCCTTCCTCTATTAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGGGGAT
CAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTAAACTATCAGATGGGCCG
AGTCCATACACGGGTGGAGAAGATTAAATCCATTTAAGTCTCAGTTTGATGATCTCATTG
ATTATCTAAGAGTTTGCCAACAAATGGCTAATTATGATATATCAGGCACCAATGTCAAGA
AACGGCTAATTATTGTCAACTTTTTTCTTTGGTCAGAGGCTCAATGTAAATTTAAAGCAT
AATAGACAAATAATATATACAAGTGTGGTTAGTTCAATCAGTTAGAAAAGATCAAATTCA
CTGATAGATCGACTCTTCACAAAGCTTCCCAGCAAAGGAGAATTTGGCAAAANAGATCA

Last Updated on 06.12.2018 16:11