Genotyping and primer details


Genotyping and primer details for 114D10 - At4g39030
Line ID 114D10
Locus At4g39030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:18188851 of line 114D10 Chr4:18188851
   
Insertion allele amplicon 3144 - 04nt
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 04nt: 5'-TTGATCTTTTATGCCAAATTCTCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:18189161
   
wt amplicon (over InsPos) 35oj - 04nt
wt amplicon start Chr4:18188197
wt amplicon end Chr4:18189161
wt amplicon size 965 bp
Locus specific primer 04nt: 5'-TTGATCTTTTATGCCAAATTCTCC-3'
2nd locus specific primer 35oj: 5'-CAACATTTGTTTCCTGTGTTTTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11