Sequences from confirmation amplicon


Confirmation sequences for line 122F10 and hit At2g16940 are shown.
Line ID 122F10
Locus At2g16940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:7346201
Primers used for PCR 49dr - 8474, show primer details
   
Sequence Name 32-K110037-0022-122-F10C-49dr
GenBank Accession LN500624 [GenBank]
Sequencing primer gene specific primer, 49dr: 5'-AAAAAGATCGGTCACGAAGAAGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K110037-0022-122-F10C-49dr
CAGGAAAGAGAAAGAGCTTGGAAATAGAACCTAAGGAAAGAGAGACCAAGGACCGGGATA
GAGATCGCAGGTGCTCCTTCTCTCTCTCTTGTATTTATTTGAAACTTGTTCTCAAAAATG
TCTACTACATTTGATCAATATACCTTTTTGTTATGATTGGTACATACTTCCTGAGAGGTA
TACATCCGAGTTGGTGATCGACTTCATAATCTATGTATTGCCTGCTAGTTCACACTTTAG
AAGCTGTGTCTTAAATTTATGTAGTGTGTTTAGTAGCTGCTTTGCCATTCTATCTTTTTC
GCATAGAGTTCTTCATGGTAAATTCAGAATATTATTGGGATGTTGTGACTGAAATTGAGG
ATGCGGGCTCCTAGTGCTTCCCGTCTCTTATGCTTTTTGGGTTATCCTACAAAAACTTTG
ATGTAAGCTATAAGTGTTAATATGGAGAAAAAGAAAGAGTAATTACCAATTTAAATAGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 16-K110036-0022-122-F10C-8409
GenBank Accession LN493472 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K110036-0022-122-F10C-8409
ATTTACTATTTAAATTGGTAATTACTCTTTCTTTTTCTCCATATTAACTCTTATAGCTTA
CATCAAAGTTTTTGTAGGATAACCCAAAAAGCATAAGAGACGGGAAGCACTAGGAGCCCG
CATCCTCAATTTCAGTCACAACATCCCAATAATATTCTGAATTTACCATGAAGAACTCTA
TGCGAAAAAGATAGAATGGCAAAGCAGCTACTAAACACACTACATAAATTTAAGACACAG
CTTCTAAAGTGTGAACTAGCAGGCAATACATAGATTATGAAGTCGATCACCAACTCGGAT
GTATACCTCTCAGGAAGTATGTACCAATCATAACAAAAAGGTATATTGATCAAATGTAGT
AGACATTTTTGAGAACAAGTTTCAAATAAATACAAGAGAGAGAGAAGGAGCACCTGCGAT
CTCTATCCCGGTCCTTGGTCTCTCTTTCCTTAGGTTCTATTTCCAAGCTCTTTTCTCTTT
CCTGACTCCGATGTCGCTCTGATCGACTTCTACTTCTTCGTGACCAATCTTTT

Last Updated on 06.12.2018 16:11