Genotyping and primer details

Genotyping and primer details for 122F10 - At2g16940
Line ID 122F10
Locus At2g16940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:7346201 of line 122F10 Chr2:7346201
Insertion allele amplicon 8474 - 49dr
Locus specific primer 49dr: 5'-AAAAAGATCGGTCACGAAGAAGTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:7346691
wt amplicon (over InsPos) 11ee - 49dr
wt amplicon start Chr2:7345866
wt amplicon end Chr2:7346691
wt amplicon size 826 bp
Locus specific primer 49dr: 5'-AAAAAGATCGGTCACGAAGAAGTA-3'
2nd locus specific primer 11ee: 5'-TAGGCCAGAGACAACATATCAGTG-3'
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11