Sequences from confirmation amplicon


Confirmation sequences for line 123D08 and hit At3g45090 are shown.
Line ID 123D08
Locus At3g45090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:16491076
Primers used for PCR 30jd - 3144, show primer details
   
Sequence Name 18-K113214-0022-123-D08C-3144
GenBank Accession LN494382 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K113214-0022-123-D08C-3144
TGACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGTTTAAACTAATATGGGG
AAGACAAGAGTTACTGGACAGACAACGAAGAAGAAGAGTCTCGAGACACAATCTCCATGG
TATCGCAAAACAACCACAACGAGGCTTCAAAGACCAACAAAGATGACAAATACTCCCAAA
ACCTCGATCTTTTCCTCAAGACAACGAACCAGCCATTGACTGAATCCCTTGAGCTCACGA
GTGAATACAGAAACAGAATGGGAGTAGCCGCCTCGGATAAAGCCAAGATGAGGAAACGTT
CACTTAGTATTCCGCCTGTCGGGAAACATGGTTCAATCATAGATGACCAGATTTTGGCTA
ACCAGACTGATTCAGTACTCTAAAGTGCCCCGTGACCTCTTTGCCTGTGGTTTTCTGGTT
TTTTTTTCTTTCCGTTTTTTTTTTTTTGGGGTTTAACCCTAAGGGGGGCCTAGGGTTCCC
AAACCCCTTGGAGGGGAAAAAAAAATTTGGCAATTTTCAAACTTTTTTGGCCCCCATAAA
AGGCCCCCTTTTTTCAACCTTGGG
   
Sequence Name 18-K113215-0022-123-D08C-30jd
GenBank Accession LN494383 [GenBank]
Sequencing primer gene specific primer, 30jd: 5'-AAAACACTAGCCCAAGAGTTGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K113215-0022-123-D08C-30jd
GAATTAGCAAATTTTGTTTTACACTTCAATGAGTTTTGGAAACCTAAGCTACCTGATAGG
TTAAAACACAAAAAAAAAAAAACGGAAAAAAAAAAAACCCCAAAACCCCCCGGGAAAAGG
GTCCCGGGGCCCTTTAAAATACCGAAACCTTCGGGTTACCCAAAATTGGGGCCTTTTTAA
TTGAACCCTTTTTCCCCAACGGGGGAAAACCAAGGGAACTTTTCCCCCTTTTGGTTTTTT
CCAAGGGGGGCACCCCCCTTTTTTTTTTTTTTTCCCCCCGGGCCCCAAGGGATTCCACCC
AGGGGGGGTTCTTTTTTTTGGAAAAAAAAACCGGGTTTTGGGAATTTTTTCCCCCTTTTT
TGGGTTTTAAACCCCCCTTGGGGTTTTTTTGCAAACCCCGGGAAATTGGGTTCCAAAACC
CCTCTTCTTCCTTTGCTGGCCCAAAACCTTTGGTTTCCCCATTTTATTTTAAACCCCCTT
TCCTTCCAAAAACCCTTCCCTTTTTTAAGGAAATTTCTTTTCTTTGGGATTGGGGCCCCC
CCCCTACCTCCGGGGAAAAA

Last Updated on 06.12.2018 16:11