Genotyping and primer details


Genotyping and primer details for 123D08 - At3g45090
Line ID 123D08
Locus At3g45090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:16491076 of line 123D08 Chr3:16491076
   
Insertion allele amplicon 3144 - 30jd
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 30jd: 5'-AAAACACTAGCCCAAGAGTTGAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:16490559
   
wt amplicon (over InsPos) 04kj - 30jd
wt amplicon start Chr3:16490559
wt amplicon end Chr3:16491656
wt amplicon size 1098 bp
Locus specific primer 30jd: 5'-AAAACACTAGCCCAAGAGTTGAAA-3'
2nd locus specific primer 04kj: 5'-GATGATGGAACAGAGCATCAACTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11