Sequences from confirmation amplicon


Confirmation sequences for line 134E03 and hit At5g60170 are shown.
Line ID 134E03
Locus At5g60170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24229340
Primers used for PCR 03bq - 8474, show primer details
   
Sequence Name 42-K107839-0022-134-E03C-03bq
GenBank Accession LN504485 [GenBank]
Sequencing primer gene specific primer, 03bq: 5'-TTCATAAGCTAGTCATGCCACATC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K107839-0022-134-E03C-03bq
ATTACAATAGAAATACTTCAGATGTGGATTATCTTTGGAACACATTACTCAAGCCAAATT
GCACATCATGTTAAGGGAAAGTTGAGATTAAACTGAACTTACAGTTGAAGGAACCTTTAT
AATTGGTTTTGCAGAAGAACTGTCACTGCAATAAGCATCCAGTGGTGGAGGTAGCATGTT
CCCTGAATGGTGCTGCAGAATATTTGTTGCTCCAGTGATTTGTTGAACTCTACTCCTTCA
AAATTACTATAAGACTTAACTAATCATCCAAAAATACTAGACCAACTATCATATTACATT
ACCTAAAGATACTGGTACCATGAAACAGAACAATCATGAAAGCAAGAGGGTATTTTAAAA
TTTCAGATGATACAAATACCTTGTATAGGCTGATATGATCTCATCTTTTGTGAAGCTATC
ATCTTGAGAACCAACCTCGTGCAAATAGAGACAATCAGGATTGACGCATGCCTGGAATAA
AAACATGCAGTTCATCACTCCAAAGACATCAACTTCAACATGCATCTGAAGCGAGGTAAT
CAAAAATAACAACGCAGAAGAACAAGAATCTATAGGAGACATACCACATTTCTCAATTGT
AAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCG
GTTATTA
   
Sequence Name 74-K107840-0022-134-E03C-8409
GenBank Accession LN513086 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K107840-0022-134-E03C-8409
TTTACAATTGAGAATGTGGTATGTCTCCTATAGATTCTTGTTCTTCTGCGTTGTTATTTT
TGATTACCTCGCTTCAGATGCATGTTGAAGTTGATGTCTTTGGAGTGATGAACTGCATGT
TTTTATTCCAGGCATGCGTCAATCCTGATTGTCTCTATTTGCACGAGGTTGGTTCTCAAG
ATGATAGCTTCACAAAAGATGAGATCATATCAGCCTATACAAGGTATTTGTATCATCTGA
AATTTTAAAATACCCTCTTGCTTTCATGATTGTTCTGTTTCATGGTACCAGTATCTTTAG
GTAATGTAATATGATAGTTGGTCTAGTATTTTTGGATGATTAGTTAAGTCTTATAGTAAT
TTTGAAGGAGTAGAGTTCAACAAATCACTGGAGCAACAAATATTCTGCAGCACCATTCAG
GGAACATGCTACCTCCACCACTGGATGCTTATTGCAGTGACAGTTCTTCTGCAAAACCAA
TTATAAAGGTTCCTTCAACTGTAAGTTCAGTTTAATCTCAACTTTCCCTTAACATGATGT
GCAATTTGGCTTGAGTAATGTGTTCCAAAGATAATCCACATCTGAAGTATTTTCTATTTG
TTAATCCTAGTTGGTGACTAACTTGATGATGTGGCATGACTAGCTTATGA

Last Updated on 06.12.2018 16:11