Genotyping and primer details


Genotyping and primer details for 134E03 - At5g60170
Line ID 134E03
Locus At5g60170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:24229340 of line 134E03 Chr5:24229340
   
Insertion allele amplicon 8474 - 03bq
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 03bq: 5'-TTCATAAGCTAGTCATGCCACATC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:24229983
   
wt amplicon (over InsPos) 14tf - 03bq
wt amplicon start Chr5:24228810
wt amplicon end Chr5:24229983
wt amplicon size 1174 bp
Locus specific primer 03bq: 5'-TTCATAAGCTAGTCATGCCACATC-3'
2nd locus specific primer 14tf: 5'-CTAGCCGATGAAGATGTATGACAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11