Sequences from confirmation amplicon


Confirmation sequences for line 145A02 and hit At5g23730 are shown.
Line ID 145A02
Locus At5g23730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:8005685
Primers used for PCR 11uv - 8474, show primer details
   
Sequence Name 70-K117671-0022-145-A02C-11uv
GenBank Accession LN512454 [GenBank]
Sequencing primer gene specific primer, 11uv: 5'-AACAAGAACAAGCACAACAACAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K117671-0022-145-A02C-11uv
CGTATCTTCCTCCTCCTCCTCCGCCTCCGACGTTATCGGAGCTATTGAATTCGATCCCAC
TGATAACATCGTCGCTACCGCCGGGATTTCAAGAAAGATTCGTTTTTACGGCCTCCCTTC
TCTTTTACGTAACAACGCTGTCTCCGGTACCGGAGTTTCCTTCGTCGATCAAGCCACCGC
CTGCGAATATTACATCTGTACTCCGGCGAAACTTAGTAGTCTCCGGTGGAGACCCGGGTC
GGGCGGTCGGGTTATTGGGTCGGGAGATTACGACGGCGTAGTGATGGAGTACGATCTTGA
GAAGAGGAAGGCGGCACGGTGGACTATATGGATCAAAGATATTCAATTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAA
   
Sequence Name 22-K117670-0022-145-A02C-8409
GenBank Accession LN496291 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K117670-0022-145-A02C-8409
CATTTACAATTGAATATCTTTGATCCATATAGTCCACCGTGCCGCCTTCCTCTTCTCAAG
ATCGTACTCCATCACTACGCCGTCGTAATCTCCCGACCCAATAACCCGACCGCCCGACCC
GGGTCTCCACCGGAGACTACTAAGTTTCGCCGGAGTACAGATGTAATATTCGCAGGCGGT
GGCTTGATCGACGAAGGAAACTCCGGTACCGGAGACAGCGTTGTTACGTAAAAGAGAAGG
GAGGCCGTAAAAACGAATCTTTCTTGAAATCCCGGCGGTAGCGACGATGTTATCAGTGGG
ATCGAATTCAATAGCTCCGATAACGTCGGAGGCGGAGGAGGAGGAGGAAGATACGACGGT
GGAGAGAGAAAGATCCCATTCGTATCTAGCTTCTTCTTGTTGTTGTGCTTGTTCTTGTTA
AAAA

Last Updated on 06.12.2018 16:11