Genotyping and primer details


Genotyping and primer details for 145A02 - At5g23730
Line ID 145A02
Locus At5g23730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:8005685 of line 145A02 Chr5:8005685
   
Insertion allele amplicon 8474 - 11uv
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 11uv: 5'-AACAAGAACAAGCACAACAACAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:8005314
   
wt amplicon (over InsPos) 44vl - 11uv
wt amplicon start Chr5:8005314
wt amplicon end Chr5:8006007
wt amplicon size 694 bp
Locus specific primer 11uv: 5'-AACAAGAACAAGCACAACAACAAG-3'
2nd locus specific primer 44vl: 5'-ACCGTTTTCGTATGACCTTGTAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11