Sequences from confirmation amplicon


Confirmation sequences for line 150E08 and hit At5g11720 are shown.
Line ID 150E08
Locus At5g11720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3779250
Primers used for PCR MR38 - 8474, show primer details
   
Sequence Name 36-K019216-022-150-E08C-8409
GenBank Accession LN502039 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K019216-022-150-E08C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGACGTTAGGTCAAGAC
ACTAAAACCTATGAGATTGATTCCCAGTTCTTGATTGGTAAAAGCATAATGGTTTCTCCA
GCGCTGAAGCAAGGGGCGGTAGCAGTGGATGCATATTTCCCTGCTGGAAATTGGTTCGAT
CTTTTTAACTATTCCTTTGCCGTTGGTGGAGATTCTGGCAAGCACGTTAGACTTGATACT
CCCGCTGATCACGTCAACGTTCATGTTCGAGAAGGTAAATAGTATATTCTTTGATAGCCA
GTTTTTAATAGCCAGAGTTGAGCTTGAACCGCGTGATTTTGGTTTAACATTGTGAATAGG
TAGCATTGGGGCTAT
   
Sequence Name 37-K019216-022-150-E08C-MR38
GenBank Accession LN502445 [GenBank]
Sequencing primer gene specific primer, MR38: 5'-CATAGCCACAATGCTACCTATTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K019216-022-150-E08C-MR38
CCAAAATCACGCGGTTCAAGCTCAACTCTGGCTATTAAAAACTGGCTATCAAAGAATATA
CTATTTACCTTCTCGAACATGAACGTTGACGTGATCAGCGGGAGTATCAAGTCTAACGTG
CTTGCCAGAATCTCCACCAACGGCAAAGGAATAGTTAAAAAGATCGAACCAATTTCCAGC
AGGGAAATATGCATCCACTGCTACCGCCCCTTGCTTCAGCGCTGGAGAAACCATTATGCT
TTTACCAATCAAGAACTGGGAATCAATCTCATAGGTTTTAGTGTCTTGACCTAACGTCAG
GATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGA
TCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTT
CGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11