Genotyping and primer details


Genotyping and primer details for 150E08 - At5g11720
Line ID 150E08
Locus At5g11720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:3779250 of line 150E08 Chr5:3779250
   
Insertion allele amplicon 8474 - MR38
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MR38: 5'-CATAGCCACAATGCTACCTATTCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:3779572
   
wt amplicon (over InsPos) AE10 - MR38
wt amplicon start Chr5:3778769
wt amplicon end Chr5:3779572
wt amplicon size 804 bp
Locus specific primer MR38: 5'-CATAGCCACAATGCTACCTATTCA-3'
2nd locus specific primer AE10: 5'-TTTGTCAAGATCCACGTTTGTAAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11