Sequences from confirmation amplicon


Confirmation sequences for line 154H11 and hit At2g29060 are shown.
Line ID 154H11
Locus At2g29060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:12482538
Primers used for PCR KT05 - 8474, show primer details
   
Sequence Name 20-K018976-022-154-H11C-KT05
GenBank Accession LN495017 [GenBank]
Sequencing primer gene specific primer, KT05: 5'-TTTTCTTGGAAGACAAGGCAGTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K018976-022-154-H11C-KT05
CCTGTGTACCTATCCCAGCCAAACGTGCTTCAAGACTGTTAGCAAAATAATGAGCCAATC
TCTCTGTTCCATCGCCGTAAGATGAAGAATGTTGCCTTATCCGACTTAACAGCTCGTCAG
CAGTTCTACGATCGTTAATCGAAACAGCTTGAGCACAAGAAACCAGCATTGTCCTCAAAT
CAGGTGTCTCTTTTGTATAACTGTTACCACTAGCTTCCGGTTTTTCGCCTTTAGGACTCT
TACTAAACGTTGAAGCTTTCGCAGGTTCCTTAGGGAAACTCTCGTTAAGAATGCATACAG
GTTGTTCCTTAGCCTCGCCAAATATCAGAATATTGTCAAACATATCAGTAAGCTCATCAG
TTTCATCAACATAAATAGCAGATTGTTTCTTACTTCTTTCTTCAGTCAAATGTTCTTCTT
CGCGCCAATGGCTCTTCTTTCCGGTTAATCTGTTAGGAACAGAGTTATCTATAACCAACT
GAGAGCTCTTAGGAAGGAATTTACTAGCTTCCTCCATCCCTTTCTTGAATTGTAACGCCA
AGTCAGTATCATTAAACATATTAGAAACCAAATCACCGCTAAAACTTGACCCGAAAACCG
ACAATATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACAT
TTTGATAAAACGACAAATTACG
   
Sequence Name 24-K018976-022-154-H11C-8409
GenBank Accession LN496806 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K018976-022-154-H11C-8409
TCCATATTGTCGGTTTTCGGGTCAAGTTTTAGCGGTGATTTGGTTTCTAATATGTTTAAT
GATACTGACTTGGCGTTACAATTCAAGAAAGGGATGGAGGAAGCTAGTAAATTCCTTCCT
AAGAGCTCTCAGTTGGTTATAGATAACTCTGTTCCTAACAGATTAACCGGAAAGAAGAGC
CATTGGCGCGAAGAAGAACATTTGACTGAAGAAAGAAGTAAGAAACAATCTGCTATTTAT
GTTGATGAAACTGATGAGCTTACTGATATGTTTGACAATATTCTGATATTTGGCGAGGCT
AAGGAACAACCTGTATGCATTCTTAACGAGAGTTTCCCTAAGGAACCTGCGAAAGCTTCA
ACGTTTAGTAAGAGTCCTAAAGGCGAAAAACCGGAAGCTAGTGGTAACAGTTATACAAAA
GAGACACCTGATTTGAGGACAATGCTGGTTTCTTGTGCTCAAGCTGTTTCGATTAACGAT
CGTAGAACTGCTGACGAGCTGTTAAGTCGGATAAGGCAACATTCTTCATCTTACGGCGAT
GGAACAGAGAGATTGGCTCATTATTTTGCTAACAGTCTTGAAGCACGTTTGGCTGGGATA
GGTACACAGGTTTATACTGCCTTGT
Primers used for PCR 19mg - 8474, show primer details
   
Sequence Name 59-K118471-0022-154-H11C-19mg
GenBank Accession LN510107 [GenBank]
Sequencing primer gene specific primer, 19mg: 5'-GAATGTTGCCTTATCCGACTTAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K118471-0022-154-H11C-19mg
ATCAGGTGTCTCTTTTGTATAACTGTTACCACTAGCTTCCGGTTTTTCGCCTTTAGGACT
CTTACTAAACGTTGAAGCTTTCGCAGGTTCCTTAGGGAAACTCTCGTTAAGAATGCATAC
AGGTTGTTCCTTAGCCTCGCCAAATATCAGAATATTGTCAAACATATCAGTAAGCTCATC
AGTTTCATCAACATAAATAGCAGATTGTTTCTTACTTCTTTCTTCAGTCAAATGTTCTTC
TTCGCGCCAATGGCTCTTCTTTCCGGTTAATCTGTTAGGAACAGAGTTATCTATAACCAA
CTGAGAGCTCTTAGGAAGGAATTTACTAGCTTCCTCCATCCCTTTCTTGAATTGTAACGC
CAAGTCAGTATCATTAAACATATTAGAAACCAAATCACCGCTAAAACTTGACCCGAAAAC
CGACAATATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATATCC
   
Sequence Name 27-K118470-0022-154-H11C-8409
GenBank Accession LN498603 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K118470-0022-154-H11C-8409
TTAGCGGTGATTTGGTTTCTAATATGTTTAATGATACTGACTTGGCGTTACAATTCAAGA
AAGGGATGGAGGAAGCTAGTAAATTCCTTCCTAAGAGCTCTCAGTTGGTTATAGATAACT
CTGTTCCTAACAGATTAACCGGAAAGAAGAGCCATTGGCGCGAAGAAGAACATTTGACTG
AAGAAAGAAGTAAGAAACAATCTGCTATTTATGTTGATGAAACTGATGAGCTTACTGATA
TGTTTGACAATATTCTGATATTTGGCGAGGCTAAGGAACAACCTGTATGCATTCTTAACG
AGAGTTTCCCTAAGGAACCTGCGAAAGCTTCAACGTTTAGTAAGAGTCCTAAAGGCGAAA
AACCGGAAGCTAGTGGTAACAGTTATACAAAAGAGACACCTGATTTGAGGACAATGCTGG
TTTCTTGTGCTCAAGCTGTTTCGATTAACGATCGTAGAACTGCTGACGAGCTGTTAAGTC
GG

Last Updated on 06.12.2018 16:11