Genotyping and primer details


Genotyping and primer details for 154H11 - At2g29060
Line ID 154H11
Locus At2g29060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:12482538 of line 154H11 Chr2:12482538
   
Insertion allele amplicon 8474 - 19mg
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 19mg: 5'-GAATGTTGCCTTATCCGACTTAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:12483033
   
Insertion allele amplicon 8474 - KT05
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KT05: 5'-TTTTCTTGGAAGACAAGGCAGTAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:12483146
   
wt amplicon (over InsPos) 02au - KT05
wt amplicon start Chr2:12482115
wt amplicon end Chr2:12483146
wt amplicon size 1032 bp
Locus specific primer KT05: 5'-TTTTCTTGGAAGACAAGGCAGTAT-3'
2nd locus specific primer 02au: 5'-ACCCATATCCTCAACAGAATCTTG-3'
   
wt amplicon (over InsPos) 20mg - 19mg
wt amplicon start Chr2:12481936
wt amplicon end Chr2:12483033
wt amplicon size 1098 bp
Locus specific primer 19mg: 5'-GAATGTTGCCTTATCCGACTTAAC-3'
2nd locus specific primer 20mg: 5'-TTGCGTTTTCTTGTGTAACAATTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11