Sequences from confirmation amplicon


Confirmation sequences for line 158E10 and hit At3g55980 are shown.
Line ID 158E10
Locus At3g55980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20778073
Primers used for PCR 06nt - 8474, show primer details
   
Sequence Name 36-K114649-0022-158-E10C-06nt
GenBank Accession LN502327 [GenBank]
Sequencing primer gene specific primer, 06nt: 5'-AAGGAAAGTTTGTTTCTTTGCTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K114649-0022-158-E10C-06nt
GTTTAGCAGCTCGGAGAAGATGCCAGGGTTGTCTCCTCTTGCTTATTCTTCAGGAGTTTC
GACTCCTCCGGTTTCTCCAATGGCTAATGGTGTTCCTTCCTCTCCAAGAAACGGCGGATC
ATGGCAGAACAGAGTCAATACCCTTACTCCACCGGCTTTGCAGCTCAATGGTGGAAGCAG
ATTGAAGTCCACACTGAGCGCTAGAGATATCGATATGGAGATGGAGATGGAATTGAGACT
CCATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 07-K114648-0022-158-E10C-8409
GenBank Accession LN489380 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K114648-0022-158-E10C-8409
CTCAATTCCATCCTCATCTCCATATCGATATCTCTAGCGCTCAGTGTGGACTTCAATCTG
CTTCCACCATTGAGCTGCAAAGCCGGTGGAGTAAGGGTATTGACTCTGTTCTGCCATGAT
CCGCCGTTTCTTGGAGAGGAAGGAACACCATTAGCCATTGGAGAAACCGGAGGAGTCGAA
ACTCCTGAAGAATAAGCAAGAGGAGACAACCCTGGCATCATCTCCAAGCTGCTAAACGGA
GACTGAGCCACGGCAGAGCCAGTTGAAGCATTAACAGGTCTCATCTCTTCGCGTTTATGA
GCAAAGAACAACNTTTTCCT

Last Updated on 06.12.2018 16:11