Genotyping and primer details


Genotyping and primer details for 158E10 - At3g55980
Line ID 158E10
Locus At3g55980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:20778073 of line 158E10 Chr3:20778073
   
Insertion allele amplicon 8474 - 06nt
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 06nt: 5'-AAGGAAAGTTTGTTTCTTTGCTCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:20777753
   
wt amplicon (over InsPos) 29oj - 06nt
wt amplicon start Chr3:20777753
wt amplicon end Chr3:20778444
wt amplicon size 692 bp
Locus specific primer 06nt: 5'-AAGGAAAGTTTGTTTCTTTGCTCA-3'
2nd locus specific primer 29oj: 5'-TAGCTGCGTTATTGTTGTTGTTTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11