Sequences from confirmation amplicon


Confirmation sequences for line 163C01 and hit At1g21380 are shown.
Line ID 163C01
Locus At1g21380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7487100
Primers used for PCR 08dr - 8474, show primer details
   
Sequence Name 17-K110359-0022-163-C01C-08dr
GenBank Accession LN493897 [GenBank]
Sequencing primer gene specific primer, 08dr: 5'-TATTCACAAGAGCCATTACACGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K110359-0022-163-C01C-08dr
CAGTCACTATAAGTTCTTCCTTCAACCCTGTCACAGCATTTAGAACAGAGAGTATTAAAA
ATCAGATCAAACAACAAAGCCATTAAACCTAATCATGTTACTATTCTACCTCTGGATGAC
TTGGATCAAGAGCCCCAAGCATGTCTGTCAAAACATCAACTGATCCCTGAGCGCTTTGAA
TCTCTTCCATGCTGAAAATGTGAAGATACTAAAGTCTATAACACGGAATACAAAATAATT
GAACAGAAAGAGAACTTGGAAATATTCAGGATATATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACAGTTATA
   
Sequence Name 01-K110358-0022-163-C01C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K110358-0022-163-C01C-8409
ATTTTTAATCCTCTCTGTTCCAAATGCTCTTACAGGGTCTGAAGGACCAACTTATATTTG
ACTTGGCTCAGCAATGTCGTCCTTATCCAAGACGTGTAATG

Last Updated on 06.12.2018 16:11