Genotyping and primer details


Genotyping and primer details for 163C01 - At1g21380
Line ID 163C01
Locus At1g21380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:7487100 of line 163C01 Chr1:7487100
   
Insertion allele amplicon 8474 - 08dr
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 08dr: 5'-TATTCACAAGAGCCATTACACGTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:7486788
   
wt amplicon (over InsPos) 01es - 08dr
wt amplicon start Chr1:7486788
wt amplicon end Chr1:7487695
wt amplicon size 908 bp
Locus specific primer 08dr: 5'-TATTCACAAGAGCCATTACACGTC-3'
2nd locus specific primer 01es: 5'-TATTGTATTGATCTTCTTTGGGGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11