Sequences from confirmation amplicon


Confirmation sequences for line 164G11 and hit At1g18370 are shown.
Line ID 164G11
Locus At1g18370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6323169
Primers used for PCR 03ie - 8474, show primer details
   
Sequence Name 41-K112875-0022-164-G11C-8409
GenBank Accession LN504141 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K112875-0022-164-G11C-8409
ATTTCATTGAATAATCCTGCATTTGACGTCAGAAGAACTGCATTTGACGTCTAAAACATT
GACTCTGACCAAAGCAGTGCTTACCTTAATTATGTGATTGTAAATATCATTGACAGCTTT
CTCCGTTACTCCTCTCATGGTATATGTCTTTCCACTGCTTGTTTGTCCATATGCAAATAT
TGTTGCTGAATCATTAAAAAGGGAAGTTAGTCATCTATGATAAATCCGAGTAAAAGACAG
AAGAATCATTAAAAAGACAAGTACCATTTATGCCCATCAAAGCAGACAATGCAACATTCT
TAACACCATCTTCATACACATTCTCTGTTAAACTCTCTGGTCCAAAAACTTTATCTGAAA
TCAAAAGAGTGCATTATTAGCCATCAAAAACCGCAGGACTTGAAGAGTTGGAAACATCAG
ATATAAAACTTCAATTACCAAATGTAAATGAGGACTGATGATGCANACGTTCTTGAACTT
GTGGTTTAGACACAATGGTGNTGGTCAT
   
Sequence Name 57-K112876-0022-164-G11C-03ie
GenBank Accession LN509470 [GenBank]
Sequencing primer gene specific primer, 03ie: 5'-AATGACCACACCATTGTGTCTAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K112876-0022-164-G11C-03ie
TCATTTGGTAATTGAAGTTTTATATCTGATGTTTCCAACTCTTCAAGTCCTGCNGGTTTT
TGATGGCTAATAATGCACTCTTTTGATTTCAGATAAAGTTTTTGGACCAGAGAGTTTAAC
AGAGAATGTGTATGAAGATGGTGTTAAGAATGTTGCATTGTCTGCTTTGATGGGCATAAA
TGGTACTTGTCTTTTTAATGATTCTTCTGTCTTTTACTCGGATTTATCATAGATGACTAA
CTTCCCTTTTTAATGATTCAGCAACAATATTTGCATATGGACAAACAAGCAGTGGAAAGA
CATATACCATGAGAGGAGTAACGGAGAAAGCTGTCAATGATATTTACAATCACATAATTA
AGGTAAGCACTGCTTTGGTCAGAGTCAATGTTTTAGACGTCAAATGCAGTTCTTCTGACG
TCAAATGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATATCCGCACGGTTAT

Last Updated on 06.12.2018 16:11