Genotyping and primer details


Genotyping and primer details for 164G11 - At1g18370
Line ID 164G11
Locus At1g18370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:6323169 of line 164G11 Chr1:6323169
   
Insertion allele amplicon 8474 - 03ie
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 03ie: 5'-AATGACCACACCATTGTGTCTAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6323649
   
wt amplicon (over InsPos) 07jm - 03ie
wt amplicon start Chr1:6322580
wt amplicon end Chr1:6323649
wt amplicon size 1070 bp
Locus specific primer 03ie: 5'-AATGACCACACCATTGTGTCTAAA-3'
2nd locus specific primer 07jm: 5'-GTATTGTCTGTTTTGCCATCAAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11