Sequences from confirmation amplicon


Confirmation sequences for line 179C04 and hit At5g05410 are shown.
Line ID 179C04
Locus At5g05410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1603039
Primers used for PCR MZ01 - 8474, show primer details
   
Sequence Name 47-K015905-022-179-C04C-8409
GenBank Accession LN506028 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K015905-022-179-C04C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGAAGCCAAAGGACCAT
ACATAGCTTTAGCAGCCTCATCATAAGCAGAAGCAGCTTCTTGAGCAGTAGGGAAAGTAC
CAAGCCAAAGCCTGCTACCTCGATTAGGCTCTCTGATCTCAGCAACCCATTTACCCCAAA
TCCTTTGCCTAACTCCTCTGAAACTACATCGGCTATTCTCTGGTCCTCCTTTACCTTTCA
TACAACCCTTCTTCGACCCTTTCGCAGGTACTTTCCTCTTCTTGGTAGAAACTTCTTCTA
CGGTCTCGTTATACTCTTTCCATCTCTTTAATCTCTCAGCCACAGTAGTACCGTCACCTC
TACTTCTAGATTTCCTTTTCCTCGATGTATCAATTTGTGTTCTGTTTCTATCTCCACTCT
GATCATAAACTGCCATCTCCTTCCCAGAAACAACACAATCTACAATACAACAACACTTCA
ATATCAGAACACAAGCTGAAACATAAGGCAAGAGAACTTAAACTTGGTTCAAAATCAGAT
CGATTTACTTATTTGTTATCAAATTAGGAACACCTAAAATTTCCCAATTTCACAGCTATA
CCTAAAAGGAAATAATCGAAAACCTAAAGACGAAGATAAACCCTAGAATCACCAATTGAC
GAGACCTAATCAGAGAAATTAATTGAAAATTTGTAAGAAATCAGAAATCAACGTACCTAT
AGGGGACGAAATTTGAAAATCAAAGTCTCTTCCTTCGAGTTGATAAATCGCTTT
   
Sequence Name 48-K015905-022-179-C04C-MZ01
GenBank Accession LN506355 [GenBank]
Sequencing primer gene specific primer, MZ01: 5'-TACGAGCAAGAAGACTAAACACGA-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K015905-022-179-C04C-mz01
CGATTTATCAACTCGAAGGAAGAGACTTTGATTTTCAAATTTCGTCCCCTATAGGTACGT
TGATTTCTGATTTCTTACAAATTTTCAATTAATTTCTCTGATTAGGTCTCGTCAATTGGT
GATTCTAGGGTTTATCTTCGTCTTTAGGTTTTCGATTATTTCCTTTTAGGTATAGCTGTG
AAATTGGGAAATTTTAGGTGTTCCTAATTTGATAACAAATAAGTAAATCGATCTGATTTT
GAACCAAGTTTAAGTTCTCTTGCCTTATGTTTCAGCTTGTGTTCTGATATTGAAGTGTTG
TTGTATTGTAGATTGTGTTGTTTCTGGGAAGGAGATGGCAGTTTATGATCAGAGTGGAGA
TAGAAACAGAACACAAATTGATACATCGAGGAAAAGGAAATCTAGAAGTAGAGGTGACGG
TACTACTGTGGCTGAGAGATTAAAGAGATGGAAAGAGTATAACGAGACCGTAGAAGAAGT
TTCTACCAAGAAGAGGAAAGTACCTGCGAAAGGGTCGAAGAAGGGTTGTATGAAAGGTAA
AGGAGGACCAGAGAATAGCCGATGTAGTTTCAGAGGAGTTAGGCAAAGGATTTGGGGTAA
ATGGGTTGCTGAGATCAGAGAGCCTAA

Last Updated on 06.12.2018 16:11