Genotyping and primer details


Genotyping and primer details for 179C04 - At5g05410
Line ID 179C04
Locus At5g05410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:1603039 of line 179C04 Chr5:1603039
   
Insertion allele amplicon 8474 - MZ01
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MZ01: 5'-TACGAGCAAGAAGACTAAACACGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:1602289
   
wt amplicon (over InsPos) FN42 - MZ01
wt amplicon start Chr5:1602289
wt amplicon end Chr5:1603318
wt amplicon size 1030 bp
Locus specific primer MZ01: 5'-TACGAGCAAGAAGACTAAACACGA-3'
2nd locus specific primer FN42: 5'-TTGCTCCTTCTGTTTCTCTTTCTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11