Sequences from confirmation amplicon


Confirmation sequences for line 184B02 and hit At1g05850 are shown.
Line ID 184B02
Locus At1g05850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1766819
Primers used for PCR 12ns - 8474, show primer details
   
Sequence Name 55-K114572-0022-184-B02C-12ns
GenBank Accession LN508884 [GenBank]
Sequencing primer gene specific primer, 12ns: 5'-GCATTCGTCCATACTGAAGGTATT-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K114572-0022-184-B02C-12ns
GACTTCACTACGGAGCAGCTGGGGAGCCCTGAAAGCTGATCTCTTGAACCACCCTGAGTA
CATTGAGCAAAACGCGACACTTGCATTCCAAGCTGCAATCTGGAGATGGATGACTCCAAT
CAAGAGAGCTCAGCCCTCAGCTCACGACATCTTTGTCGGAAACTGGAAACCTACAAAGAA
CGACACTTTGTCCAAGCGTGGCCCGACTTTTGGCAGCACCATGAACGTCCTCTACGGAGA
GTACACATGTGGTCAAGGTTCCATTGATCCAATGAACAACATAATCTCACACTACTTATA
CTTCCTTGACCTCATGGGTATTGGAAGAGAAGACGCGGGACCAAACGATGAGCTCAGCTG
CGCAGAACAGAAACCTTTCAACCCTTCAACTGTACCTTCCTCTTCCTCTTCGTAAGTCTT
ATGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAA
TGTA
   
Sequence Name 43-K114571-0022-184-B02C-8409
GenBank Accession LN504926 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K114571-0022-184-B02C-8409
CATTTACATTGAATATATCATAAGACTTACGAAGAGGAAGAGGAAGGTACAGTTGAAGGG
TTGAAAGGTTTCTGTTCTGCGCAGCTGAGCTCATCGTTTGGTCCCGCGTCTTCTCTTCCA
ATACCCATGAGGTCAAGGAAGTATAAGTAGTGTGAGATTATGTTGTTCATTGGATCAATG
GAACCTTGACCACATGTGTACTCTCCGTAGAGGACGTTCATGGTGCTGCCAAAAGTCGGG
CCACGCTTGGACAAAGTGTCGTTCTTTGTAGGTTTCCAGTTTCCGACAAAGATGTCGTGA
GCTGAGGGCTGAGCTCTCTTGATTGGAGTCATCCATCTCCAGATTGCAGCTTGGAATGCA
AGTGTCGCGTTTTGCTCAATGTACTCAGGGTGGTTCAAGAGATCAGCTTTCAGGGCTTCC
CCAGCTGCTCCGTAGTTGAAGTTCCTTCAAATAACACACAGGTTAAAATACCTTCAGTAT
GAACGAATG

Last Updated on 06.12.2018 16:11