Genotyping and primer details


Genotyping and primer details for 184B02 - At1g05850
Line ID 184B02
Locus At1g05850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:1766819 of line 184B02 Chr1:1766819
   
Insertion allele amplicon 8474 - 12ns
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 12ns: 5'-GCATTCGTCCATACTGAAGGTATT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:1767296
   
wt amplicon (over InsPos) 03oe - 12ns
wt amplicon start Chr1:1766290
wt amplicon end Chr1:1767296
wt amplicon size 1007 bp
Locus specific primer 12ns: 5'-GCATTCGTCCATACTGAAGGTATT-3'
2nd locus specific primer 03oe: 5'-TCCACCAATTCCCGTATTTTATTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11