Sequences from confirmation amplicon


Confirmation sequences for line 215B10 and hit At1g50410 are shown.
Line ID 215B10
Locus At1g50410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:18676588
Primers used for PCR HP14 - 8474, show primer details
   
Sequence Name 59-K017630-022-215-B10C-HP14
GenBank Accession LN509852 [GenBank]
Sequencing primer gene specific primer, HP14: 5'-CCTAGCTGCTTGTTCTGATTTTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K017630-022-215-B10C-HP14
ATAAATTCTCTATTATTTTCTCTTTGCAGGATCCACCAGAAGACCCTGTTGTTACTTTGT
GTGGCCATATATTCTGCTATCAGTGTGTATCAGATTATATCACAGGGGATGAGGACACGT
GCCCTGCGCCTAGATGCAGAGAACAGCTTGCGCATGATGTTGTTTTCTCTAAATCCACGC
TTAGAAGTTGCGTTGCTGATGATTTGGGTTGTAGTTCCTCAGAGGATAATAGTCATGATA
AATCAGTTTTTCAGAATGGCGAGTTTAGTTCATCAAAGATCAAAGCTGTCTTAGATATTC
TGCAGTCGCTTTCTAACCAAGGCACTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTG
ATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCT
AATTCGGAAATCTTTATTTCGACGTGTCTACATTC
   
Sequence Name 63-K017630-022-215-B10C-8409
GenBank Accession LN510875 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K017630-022-215-B10C-8409
ACATGAAGCCATTTACAATTGAAGTGCCTTGGTTAGAAAGCGACTGCAGAATATCTAAGA
CAGCTTTGATCTTTGATGAACTAAACTCGCCATTCTGAAAAACTGATTTATCATGACTAT
TATCCTCTGAGGAACTACAACCCAAATCATCAGCAACGCAACTTCTAAGCGTGGATTTAG
AGAAAACAACATCATGCGCAAGCTGTTCTCTGCATCTAGGCGCAGGGCACGTGTCCTCAT
CCCCTGTGATATAATCTGATACACACTGATAGCAGAATATATGGCCACACAAAGTAACAA
CAGGGTCTTCTGGTGGATCCTGCAAAGAGAAAATAAAACGGAATTTATGGGTAAAGAAAA
TCAGAACAAGCAGCT

Last Updated on 06.12.2018 16:11