Genotyping and primer details


Genotyping and primer details for 215B10 - At1g50410
Line ID 215B10
Locus At1g50410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:18676588 of line 215B10 Chr1:18676588
   
Insertion allele amplicon 8474 - HP14
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer HP14: 5'-CCTAGCTGCTTGTTCTGATTTTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:18676229
   
wt amplicon (over InsPos) 04si - HP14
wt amplicon start Chr1:18676229
wt amplicon end Chr1:18677080
wt amplicon size 852 bp
Locus specific primer HP14: 5'-CCTAGCTGCTTGTTCTGATTTTCT-3'
2nd locus specific primer 04si: 5'-GCCTGATCTTCAGTAGTTGGATTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11