Sequences from confirmation amplicon


Confirmation sequences for line 225G05 and hit At1g74640 are shown.
Line ID 225G05
Locus At1g74640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28033128
Primers used for PCR 08op - 8474, show primer details
   
Sequence Name 52-K114782-0022-225-G05C-08op
GenBank Accession LN507902 [GenBank]
Sequencing primer gene specific primer, 08op: 5'-TTCTGGTTCAAAAGTCTCAACCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K114782-0022-225-G05C-08op
CCCTAATTTTTTGTATAATTTCGCTTCCATGGCTGCTTCAGCTGCATTCTCCATTTCATC
ACCACCTTTTGTACCCTTTACCACCAAAATTCGCAGACCATTTCTCTTGCTTACTCCAAA
ATATCCCAAACCTGTTTCGGTGCGAGCTTCTGTGTCCTCGATCTCCATCGATGAAAAATT
GCCAGTCCAAACAAAGGTTAGTTTACTAGTATCTTGTTTTGTATTAATGGTTTGATCCTT
TGACTTGGAGAATGAATTAGACAAAATTGTATTAGTCATGTTGAGATTCTATTGAGTTTT
GTTTCACATCAATTGATATAGCCAGGATATATTCACATCAAAGCCGTGCAGGATATATTC
AATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
GC
   
Sequence Name 52-K114769-0022-225-G05C-8409
GenBank Accession LN507901 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K114769-0022-225-G05C-8409
AAAACTTAATCGAAATCTCAACATGAACTAAATACAATTTTCGTTCTAATTCCATTCCTC
CAAGTCAAAGGGATCAAACCATTAATACAAAACAAGATACTAGTAAACTCAACCTTTGTT
TGGAACTGGCAATTTTTTCATCGATGGAGATTCGAGGACACAGAAGCTCGCACCGAAACA
GGTTTGGGATATTTTCGGAGTAAGCAAGAGAAATCGGTCTGCGAATTTTGGTGGTAAAGG
GTACAAAAGGTGGTGATGAAATGGAGAATGCAGCTTAAGCAGCCATGGAAGCGAAATTTA
TACAAAAAATTAGGGTTTTTTCGAAGATCTGAAGCGAGAAAGAAGGAAACGACGATTCAA
AGGTTT

Last Updated on 06.12.2018 16:11