Genotyping and primer details


Genotyping and primer details for 225G05 - At1g74640
Line ID 225G05
Locus At1g74640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:28033128 of line 225G05 Chr1:28033128
   
Insertion allele amplicon 8474 - 08op
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 08op: 5'-TTCTGGTTCAAAAGTCTCAACCTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:28032754
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 31.05.2018 12:55