Genotyping and primer details

Genotyping and primer details for 225G05 - At1g74640
Line ID 225G05
Locus At1g74640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:28033128 of line 225G05 Chr1:28033128
Insertion allele amplicon 8474 - 08op
Locus specific primer 08op: 5'-TTCTGGTTCAAAAGTCTCAACCTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:28032754
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 14:50