Sequences from confirmation amplicon


Confirmation sequences for line 236E05 and hit At4g31900 are shown.
Line ID 236E05
Locus At4g31900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15432161
Primers used for PCR 05ef - 8474, show primer details
   
Sequence Name 32-K110678-0022-236-E05C-05ef
GenBank Accession LN500629 [GenBank]
Sequencing primer gene specific primer, 05ef: 5'-ACAATATCTTGTGAAGTGGAAGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K110678-0022-236-E05C-05ef
GTATTCCAGCAAATGCGATATCGTGTTGTATTTAATTAACATGTCGATTATTATGTTTTT
GTTGCTTCTCTTGTCTGGTTATCTTATCATCACTTGATTATTAATGTCAATTCTTAGGGT
GCCCGAGCAGGAGTTCGAGAAGGCTTACAAGTCCCATCCTCATTTAAAACTAAAATTAAG
GGTGACCAGGTTTAACGCTGCTATGGACGTATTCATCGCTGAGAACGGAGCTCATGAGTT
TATCGCCATTCGTCCTGAATGGAAAACTGTTGACCGGATTATTGCAGGATATATTCAATT
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAGATGTCCGCAC
AATTA
   
Sequence Name 16-K110677-0022-236-E05C-8409
GenBank Accession LN493477 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K110677-0022-236-E05C-8409
CATTTACATTGAATTATCCTGCAATAATCCGGTCAACAGTTTTCCATTCAGGACGAATGG
CGATAAACTCATGAGCTCCGTTCTCAGCGATGAATACGTCCATAGCAGCGTTAAACCTGG
TCACCCTTAATTTTAGTTTTAAATGAGGATGGGACTTGTAAGCCTTCTCGAACTCCTGCT
CGGGCACCCTAAGAATTGACATTAATAATCAAGTGATGATAAGATAACCAGACAAGAGAA
GCAACAAAAACATAATAATCGACATGTTTAATTAAATACAACACGATATCGCATATTGCT
GGGAATTTACCAAGAGCAGTGCAAGTACGATAACCCCTTCCACTTCACAAGATATTG

Last Updated on 06.12.2018 16:11