Genotyping and primer details


Genotyping and primer details for 236E05 - At4g31900
Line ID 236E05
Locus At4g31900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:15432161 of line 236E05 Chr4:15432161
   
Insertion allele amplicon 8474 - 05ef
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 05ef: 5'-ACAATATCTTGTGAAGTGGAAGGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:15431824
   
wt amplicon (over InsPos) 01fd - 05ef
wt amplicon start Chr4:15431824
wt amplicon end Chr4:15432558
wt amplicon size 735 bp
Locus specific primer 05ef: 5'-ACAATATCTTGTGAAGTGGAAGGG-3'
2nd locus specific primer 01fd: 5'-TGAATTCCTCTCGATTTCTCTCAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11