Sequences from confirmation amplicon


Confirmation sequences for line 242G08 and hit At1g05360 are shown.
Line ID 242G08
Locus At1g05360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1566881
Primers used for PCR CP82 - 8474, show primer details
   
Sequence Name 11-K105564-0022-242-G08C-CP82
GenBank Accession LN491110 [GenBank]
Sequencing primer gene specific primer, CP82: 5'-AAAAGTAACAAACCTCGGACATTG-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K105564-0022-242-G08C-CP82
CAATTTCAAATTGATTATGGAAAATTAATCAATTTATTTCAACCATATAACATACATCAA
GAGGTGAAACAAATCACATTTATAACTTTAGTTAGAACTTCCTTTCAACTTTCAATTGTT
TTTCTATGTTTTTTTGTTCTTTCTGGACAAATTTGTTATGTTCTCAGATAAGACGAAATA
CAGTGATAATGTTTCTATGAAAGAAAGAAAAAAAAACCAAAGTTAGAAAAGATAAATAAA
TTTCTGGACTACTTAGATTTCTTTGAGGTCAGCGACGACTTGTTGGTCAAGGCATCTAGT
TCTTCTTCTTGTTGCTTCTTTAGGTATCTCTGTGCAGTTGAAGTCACAATCTGGCCGAAG
AAATTCAAAAGCATCAGCCACACGACTCCGTTCCACACGGATGCAAAAGAAAGATCCCAT
TTCTTGACCTGAACCAAGAAAAAAATCATCCAAATACCTCAAAATGCAGAACAATGCAAA
ACGGTAATGGACTAAGTAAAGAGAAACAAAAGATACGTTACATTGATATCAGAAGATACT
GGAGGCGAAGCAATCAAGTACTTCTCTTTCATTAAGCGGAGTTTCGCTGTGAGCTCAGGC
AATGCAGAAGCAAAACCGGGCACAAAGCTCAGAATATATATCAGCTCATATATCTCAATT
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATT
   
Sequence Name 59-K105565w-0022-242-G08C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K105565w-0022-242-G08C-8409
TTGAGATATATGAGCTGATATATATTCTGAGCTTTGTGCCCGGTTTTGCTTCTGCATTGC
CTGAGCTCACAGCGAAACTCCGCTTAATGAAAGAGAAGTACTTGATTGCTTCGCCTCCAG
TATCTTCTGATATCAATGTAACGTATCTTTTGTTTCTCTTTACTTAGTCCATTACCGTTT
TGCATTGTTCTGCATTTTGAGGTATTTGGATGATTTTTTTCTTGGTTCAGGTCAAGAAAT
GGGATCTTTCTTTTGCATCCGTGTGGAACGGAGTCGTGTGGCTGATGCTTTTGAATTTCT
TCGGCCAGATTGTGACTTCAACTGCACAGAGATACCTAAAGAAGCAACAAGAAGAAGAAC
TAGATGCCTTGACCAACAAGTCGTCGCTGACCTCAAAGAAATCTAAGTAGTCCAGAAATT
TATTTATCTTTTCTAACTTTGGTTTTTTTTTCTTTCTTTCATAGAAACATTATCACTGTA
TTTCGTCTTATCTGAGAACATAACAAATTTGTCCAGAAAGAACAAAAAAACATAGAAAAA
CAATTGAAAGTTGAAAGGAAGTTCTAACTAAAGTTATAAATGTGATTTGTTTCACCTCTT
GATGTATGTTATATGGTTGAAATAAATTGATTAATTTTCCATAATCAATTTTGAAATTTG
TTCTGATACTAAAGAATATCTTCAATGTCC
Primers used for PCR AR14 - 8474, show primer details
   
Sequence Name 29-K118837-0022-242-G08C-AR14
GenBank Accession LN499498 [GenBank]
Sequencing primer gene specific primer, AR14: 5'-AATCCAATGCTTCTTGCATAGACT-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K118837-0022-242-G08C-AR14
ATGTGCTTATTACATTACGTTCTGCCTTACATTCAGGTTCCTAACCCATTATTCGACCTT
GCTGGAATCATGTGTGGACAATTCGAGAAACCGTTTTGGGAGTTTTTCCTTGCAACCTTG
ATTGGAAAGGCAATCATCAAGACACATATACAGGTTTTGTGAGCGTTTCTGTAATACCCT
ATTTGTTTTGAGTCACTTTCGTTCTCATTCTTTGGGTTTCATCTCATTGTCAACATTTTA
TCTTTTGCAGACGGTTTTCATCATATGCGTCTGTAATAATCAGCTTCTTGACTGGGTAGA
GAATGAAAGAACCAATTTACCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCCACACGGTTATTA
   
Sequence Name 05-K118836-0022-242-G08C-8409
GenBank Accession LN488570 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K118836-0022-242-G08C-8409
CATTTACATTGGGTAAATTGGTTCTTTCATTCTCTACCCAGTCAAGAAGCTGATTATTAC
AGACGCATATGATGAAAACCGTCTGCAAAAGATAAAATGTTGACAATGAGATGAAACCCA
AAGAATGAGAACGAAAGTGACTCAAAACAAATAGGGTATTACAGAAACGCTCACAAAACC
TGTATATGTGTCTTGATGATTGCCTTTCCAATCAAGGTTGCAAGGAAAAACTCCCAAAAC
GGTTTCTCGAATTGTCCACACATGATTCCAGCAAGGTCGAATAATGGGTTAGGAACCTGA
ATGTAAGGCAGAACGTAATTGTAATAAGCACATCATAAGTTTCCCGGAACCTTAAACACA
CACAAAAAGTCTATGCAAGAACCATTGGAT

Last Updated on 06.12.2018 16:11