Genotyping and primer details


Genotyping and primer details for 242G08 - At1g05360
Line ID 242G08
Locus At1g05360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:1566881 of line 242G08 Chr1:1566881
   
Insertion allele amplicon 8474 - AR14
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AR14: 5'-AATCCAATGCTTCTTGCATAGACT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:1566517
   
Insertion allele amplicon 8474 - CP82
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CP82: 5'-AAAAGTAACAAACCTCGGACATTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:1567574
   
wt amplicon (over InsPos) AR14 - CP82
wt amplicon start Chr1:1566517
wt amplicon end Chr1:1567574
wt amplicon size 1058 bp
Locus specific primer CP82: 5'-AAAAGTAACAAACCTCGGACATTG-3'
2nd locus specific primer AR14: 5'-AATCCAATGCTTCTTGCATAGACT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11