Sequences from confirmation amplicon


Confirmation sequences for line 245D11 and hit At1g11160 are shown.
Line ID 245D11
Locus At1g11160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3733635
Primers used for PCR RH11 - 8474, show primer details
   
Sequence Name 28-K117387-0022-245-D11C-RH11
GenBank Accession LN499020 [GenBank]
Sequencing primer gene specific primer, RH11: 5'-AAAACAATGCACTTGTCGTTACTG-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K117387-0022-245-D11C-RH11
TACCCAACGACCATCAGGGCTAAATTCAATAGTGCTGATGCCGCGAGTATGACCCTTGTA
TGTTTGGATGCATCCCTTCTTTCTGGTATCCCAAACCCTTAGATTGGTGTCACTAGATCC
CGATGCAAGAAATTCACCAAATGGATGAAACTCAACAGCAGAACAATTGGATCTGTGTCC
AGTAAAAGCTCGAACCACTACACTTCAAGAGCAAGAGTGAAAATATTAGACCTCATTATC
TGAAACTAACCATCCATCACAGGACTTATAATTCGTCCAGACAATAACAAGTGTGAGTGC
CTCTAATATAATCTAGGGATAATATATGAGAAGAGAAAATTAATCAGCTTACTCTTTGAT
TCTTCTAGATCCCACAGCTTTATCACACCTGAAGAAGCCCCAGCGAGTACCAAAACTTCT
TCTGAGTTAAAAGCTACTGAATCAACCGGGCTTGTATGCCCACAAAGACTCTGCCAACAA
CACATAAAATAAAATATCATCACTCTTCTATGAAGAGAAACAAGATGTTACATGCTTTAT
ACCAAGAGGGACCACAATTTTGACATTTTCATACACATTAGCTGAAATAACTGTAGTTAT
AGCTATAAGTGGCAAATCTTGAAACCAGATAAGAAATTGCAAATGAAGACCAGCGAGAAT
GAACAAATTAAAAAAAGAGAGAAATGTGAAAGAGACCATTGGAGAAGTGGTTTTGCCGAT
TGACCAAAGGTTGACCTTATAATCATCTCCACCTGTTAGAAGGAGCCGTGACGTCTTCTT
TCCGATACTTAGACAATTCACATTTCCAGAATGTGCCACAAACTCCTCTAATTTTCCAAA
TCCAAGTCAAGAAAAATTCATATCCNGAACGAAAGAAAAAGTCAAGACAAACAGAGANTA
ACATGTCTCTTCTATCATCTGAACTCGGTTTGGGTTTTTAGATGACAGAGGCAGAAATTA
GAACCCAGAAGAACATTTTCATGAAAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAGAGTAT
   
Sequence Name 01-K117386-0022-245-D11C-8409
GenBank Accession LN486753 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K117386-0022-245-D11C-8409
CATTTTTCTGAAATGTTCTTCTGGGTTCTAATTTCTGCCTCTGTCATCTTAAAAACCAAA
CCGAGTTCAGATGATAGAAGAGACATTGTTATCTCTGTTTGTCTTGACTTTTTCTTTCGT
TCTGGATATGAATTTTTCTTGACTTGGATTTGGAAAATTAGAGGAGTTTGTGGCACATTC
TGGAAATGTGAATTGTCTAAGTATCGGAAAGAAGACGTCACGGCTCCTTCTAACAGGTGG
AGATGATTATAAGGTCAACCTTTGGTCAATCGGCAAAACCACTTCTCCAATGGTCTCTTT
CACATTTCTCTCTTTTTTTAATTTGTTCATTCTCGCTGGTCTTCATTTGCAATTTCTTAT
CTGGTTTCAAGATTTGCCACTTATAGCTATAACTACAGTTATTTCAGCTAATGTGTATGA
AAATGTCAAAATTGTGGTCCCTCTTGGTATAAAGCATGTAACATCTTGTTTCTCTTCATA
GAAGAGTGATGATATTTTATTTTATGTGTTGTTGGCAGAGTCTTTGTGGGCATACAAGCC
CGGTTGATTCAGTAGCTTTTAACTCAGAAGAAGTTTTGGTACTCGCTGGGGCTTCTTCAG
GTGTGATAAAGCTGTGGGATCTAGAAGAATCAAAGAGTAAGCTGATTAATTTTCTCTTCT
CATATATTATCCCTAGATTATATTAGAGGCACTCACACTTGTTATTGTCTGGACGAATTA
TAAGTCCTGTGATGGATGGTTAGTTTCAGATAATGAGGTCTAATATTTTCACTCTTGCTC
TTGAAGTGTAGTGGTTCGAGCTTTTACTGGACACAGATCCAATTGTTCTGCTGTTGAGTT
TCATCCATTTGGTGAATTTCTTGCATCGGGATCTAGTGACACCAATCTAAGGGTTTGGGA
TACCAGAAAGAAGGGATGCATCCAAACATACAAGGGTCATACTCGCGGCATCAGCACTAT
TGAAATTAGCCCTGATGGTCGTTGGGTAGTGTCTGGAGGACTTGACAACG

Last Updated on 06.12.2018 16:11