Genotyping and primer details


Genotyping and primer details for 245D11 - At1g11160
Line ID 245D11
Locus At1g11160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:3733635 of line 245D11 Chr1:3733635
   
Insertion allele amplicon 8474 - RH11
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer RH11: 5'-AAAACAATGCACTTGTCGTTACTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:3734687
   
wt amplicon (over InsPos) 30vc - RH11
wt amplicon start Chr1:3733549
wt amplicon end Chr1:3734687
wt amplicon size 1139 bp
Locus specific primer RH11: 5'-AAAACAATGCACTTGTCGTTACTG-3'
2nd locus specific primer 30vc: 5'-GATGTTGTTCATTCACTGTAGCGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11