Sequences from confirmation amplicon


Confirmation sequences for line 247H11 and hit At3g12070 are shown.
Line ID 247H11
Locus At3g12070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3845913
Primers used for PCR AB10 - 8474, show primer details
   
Sequence Name 21-K024603-022-247-H11C-8409
GenBank Accession LN495503 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K024603-022-247-H11C-8409
AGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGGTGGAAGAGTTTAACGC
TTACATCAGGGAGTTTCTCAGGTCGTCCATTTAAACCTCCAGACTCGTAGTCTTGTCTTT
CACATAACCACCATCCAAGTAAGTCCTTATCAACACGATGGAGATTCCCGGTGATAGCAA
GAGCACCCACACAACAGAAAACTGCAGCAACATAAAGAATCAATGCCCTTTAATTACCAT
ATGATGCTGCAATGATATTCAGTAAAGTCATCTAGACACATATTGAATGATGATCCCAGG
GTTAAAAAGTTAAATTAGCATGAACATACTCTGTCCTGCATGAGACTCCGCTCCAGGTGA
GCACCCAAAACCACCATCCAGGTTTTTGCAGCTCACAATGTAATCAACAGCCTT
   
Sequence Name 22-K024603-022-247-H11C-AB10
GenBank Accession LN495950 [GenBank]
Sequencing primer gene specific primer, AB10: 5'-CAATGTGAAGAAGGCTGTTGATTA-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K024603-022-247-H11C-AB10
CATTGTGAGCTGCAAAAACCTGGATGGTGGTTTTGGGTGCTCACCTGGAGCGGAGTCTCA
TGCAGGACAGAGTATGTTCATGCTAATTTAACTTTTTAACCCTGGGATCATCATTCAATA
TGTGTCTAGATGACTTTACTGAATATCATTGCAGCATCATATGGTAATTAAAGGGCATTG
ATTCTTTATGTTGCTGCAGTTTTCTGTTGTGTGGGTGCTCTTGCTATCACCGGGAATCTC
CATCGTGTTGATAAGGACTTACTTGGATGGTGGTTATGTGAAAGACAAGACTACGAGTCT
GGAGGTTTAAATGGACGACCTGAGAAACTCCCTGATGTAAGCGTTAAACTCTTCCACCAG
GATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGA
TCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTT
CGACGTGTCTACA

Last Updated on 06.12.2018 16:11