Genotyping and primer details


Genotyping and primer details for 247H11 - At3g12070
Line ID 247H11
Locus At3g12070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:3845913 of line 247H11 Chr3:3845913
   
Insertion allele amplicon 8474 - AB10
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AB10: 5'-CAATGTGAAGAAGGCTGTTGATTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:3846292
   
wt amplicon (over InsPos) 08sq - AB10
wt amplicon start Chr3:3845489
wt amplicon end Chr3:3846292
wt amplicon size 804 bp
Locus specific primer AB10: 5'-CAATGTGAAGAAGGCTGTTGATTA-3'
2nd locus specific primer 08sq: 5'-TCCAGGATACTCGAGAAGAGAGAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11