Sequences from confirmation amplicon


Confirmation sequences for line 250C04 and hit At5g05780 are shown.
Line ID 250C04
Locus At5g05780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1737701
Primers used for PCR 07gz - 8474, show primer details
   
Sequence Name 29-K112307-0022-250-C04C-07gz
GenBank Accession LN499355 [GenBank]
Sequencing primer gene specific primer, 07gz: 5'-TGTGCCGTGAGAGAGAGTAAAGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K112307-0022-250-C04C-07gz
GAGCGCATTACCTCCGAATGAGGGATGATAGATAGATAACGAGCATCATGTCATTTGTTT
TCACTGCACATTATAAATACAGTAAAGATTAATGAAGCAGATCTAAGAAAGCAGCCCAAT
ATAATATCAAAATGATAGCAGAAACTCACCGGAGAAGGCCTTGACCAACTCGTTCACATT
CAGGTTAGGAAGCAAGTTGAAAACGTCCTGTCGATTTTACGAGAATATATTTCATTAGAC
GAAATCAACCAACAGCAGAGACAGTGACTAGAATATTTTAAAGAGCGCAAAACACATAGG
TTTGGAGAAAAAGAAATTATAAATCTACATAATTGTAAATATTCAATTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
   
Sequence Name 13-K112306-0022-250-C04C-8409
GenBank Accession LN492104 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K112306-0022-250-C04C-8409
TCATTGAAATTTACAATTATGTAGATTTATAATTTCTTTTTCTCCAAACCTATGTGTTTT
GCGCTCTTTAAAATATTCTAGTCACTGTCTCTGCTGTTGGTTGATTTCGTCTAATGAAAT
ATATTCTCGTAAAATCGACAGGACGTTTTCAACTTGCTTCCTAACCTGAATGTGAACGAG
TTGGTCAAGGCCTTCTCCGGTGAGTTTCTGCTATCATTTTGATATTATATTGGGCTGCTT
TCTTAGATCTGCTTCATTAATCTTTACTGTATTTATAATGTGCAGTGAAAACAAATGACA
TGATGCTCGTTATCTATCTATCATCCCTCATTCGGAGTGTAATTGCGCTCCACAACTTGA
TCAACAACAAGGTTAGATCTTTACTTTACTCTCTCTCACGGCAC

Last Updated on 06.12.2018 16:11