Genotyping and primer details


Genotyping and primer details for 250C04 - At5g05780
Line ID 250C04
Locus At5g05780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:1737701 of line 250C04 Chr5:1737701
   
Insertion allele amplicon 8474 - 07gz
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 07gz: 5'-TGTGCCGTGAGAGAGAGTAAAGTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:1738063
   
wt amplicon (over InsPos) 09hv - 07gz
wt amplicon start Chr5:1737220
wt amplicon end Chr5:1738063
wt amplicon size 844 bp
Locus specific primer 07gz: 5'-TGTGCCGTGAGAGAGAGTAAAGTA-3'
2nd locus specific primer 09hv: 5'-GTGTTTTGTGATTGTTCTGAGGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11