Sequences from confirmation amplicon


Confirmation sequences for line 272B11 and hit At3g07720 are shown.
Line ID 272B11
Locus At3g07720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2466446
Primers used for PCR CQ17 - 8474, show primer details
   
Sequence Name 65-K105460-0022-272-B11C-CQ17
GenBank Accession LN511403 [GenBank]
Sequencing primer gene specific primer, CQ17: 5'-ACTGATGGAGGAGGGTTTAATTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K105460-0022-272-B11C-CQ17
TAGACTATGACTTTGTCATTATTCAATTGACTAGTATTTGTGAATGCTGACTCAACTCTT
TGTTTCTTTCTCATTGATTTCTGCTAATACTTTTTGTAATGTTGATTATAAAAACATTGT
AAGATTTAGTATTTGAGTAATTTATGTTTTGTGTTATTTGTCATTCAGCTTAAACAGAAA
GGAACCGGACCAGGAGCAAGAAGCTCTCATGCTATAGCTCTGGTGGGAAACAAAATGTAT
GCCTTTGGTGGCGAGTTTCAACCACGTGTTCCTGTTGACAACCAGCTCTACGTTTTCGAC
CTCGAAACTCAAACATGGTCTATCCGGGAAGCTTCTGGGGATGCTCCTCCTCCTAGAGTA
GGTGTAGCCATGGCTGCAGTCGGACCCATCATCTATTTCTTTGGTGGCCGGGACTCGACT
CACCAGGAGCTCAACGAGTTATACTGTTTTAACACTTTGACCAACCAGTGGAAACTACTC
TCTTCAGGGGAAACCGGTCCACAGAACCGAAGCTACCACTCTATTACTGCGGATTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 68-K105461-0022-272-B11C-8409
GenBank Accession LN512017 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K105461-0022-272-B11C-8409
CCCTTTCAATCCGCAGATAATAGAGTGGCTAGCTTCGGTTCTGTGGACCGTGTTTCCCCT
GAAGAGAGTAGCTTTCCACTGGTTTGGTCAAAGTGTTAAAACAGTATAACTCGTTGAGCT
CCTGGTGAGTCGAGTCCCGGCCACCAAAGAAATAGATGATGGGTCCGACTGCAGCCATGG
CTACACCTACTCTAGGAGGAGGAGCATCCCCAGAAGCTTCCTGGATAGACCATGTCTGAG
TTTCGAGGTCGAAAACGTAGAGCTGGTTGTCAACAGGAACACGTGGTTGAAACTCGCCAC
CAAAGGCATACATTTTGTTTCCCACAAGAGCTATAGCATGAGAGCTTCTTGCTCCTGGTC
CGGTTCCTTTCTGTTTAAGCTGAATGACAAATAACACAAAACATAAATTACTCAAATACT
AAATCTTACAATGTTTTTATAATCAACATTACAAAAAGTATTAGCAGAAATCAATGAGAA
AGAAACAAAGAGTTGAGTCAGCATTCACAAATACTAGTCAATTGAATAATGACAAAGTCA
TAGTCTACTAATCCAATACTTATATGTGATGATGATTACAAAATTAAACCCTCCGCCATC
A

Last Updated on 06.12.2018 16:11