Sequences from confirmation amplicon


Confirmation sequences for line 278A03 and hit At3g07720 are shown.
Line ID 278A03
Locus At3g07720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2465447
Primers used for PCR 23fr - 8474, show primer details
   
Sequence Name 48-K111327-0022-278-A03C-23fr
GenBank Accession LN506533 [GenBank]
Sequencing primer gene specific primer, 23fr: 5'-AAAATTAAACCCTCCTCCATCAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K111327-0022-278-A03C-23fr
GACACTCTGCCGGTGAATAATCCATAGTCCATTCAATAAATGACAAACTCATCATCCACA
AATCCAAAATTGATCAGAGAAAGCATAAACTAGGAGATTCGTTGTAGAACCAAAATGATT
ATTGAACCTAAGTGTATAATCACATCAGCAATCTAACAGAGAACTTGGACAGATTTACAG
AGAATAATAAACAGATTCCAAAACGAAATTAGAATTTCAAAAGCCGAAAGTCTCGTACGA
TTCAATAAAAATCCGAAAACATGAGACAAGTAATATCCGATGAAACGAGATTCGTCTCTA
AGAGAAACACAAAGGCGAGAAAGCTGACCTGAACCCATTTGCCTTCCATTGGAGTCGCTG
CCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGGATTTATTAT
   
Sequence Name 40-K111326-0022-278-A03C-8409
GenBank Accession LN503745 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K111326-0022-278-A03C-8409
GACTCCATGGAAGGCAATGGGTTCAGGTCAGCTTTCTCGCCTTTGTGTTTCTCTTAGAGA
CGAATCTCGTTTCATCGGATATTACTTGTCTCATGTTTTCGGATTTTTATTGAATCGTAC
GAGACTTTCGGCTTTTGAAATTCTAATTTCGTTTTGGAATCTGTTTATTATTCTCTGTAA
ATCTGTCCAAGTTCTCTGTTAGATTGCTGATGTGATTATACACTTAGGTTCAATAATCAT
TTTGGTTCTACAACGAATCTCCTAGTTTATGCTTTCTCTGATCAATTTTGGATTTGTGGA
TGATGAGTTTGTCATTTATTGAATGGACTATGGATTATTCACCGGCAGAGTTGTTCATAT
CATGTTGATTAACAGTTTACTGATGGAGGAGGGTTTAATTT

Last Updated on 06.12.2018 16:11