Genotyping and primer details


Genotyping and primer details for 278A03 - At3g07720
Line ID 278A03
Locus At3g07720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:2465447 of line 278A03 Chr3:2465447
   
Insertion allele amplicon 8474 - 23fr
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 23fr: 5'-AAAATTAAACCCTCCTCCATCAGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:2465850
   
wt amplicon (over InsPos) 01gf - 23fr
wt amplicon start Chr3:2465054
wt amplicon end Chr3:2465850
wt amplicon size 797 bp
Locus specific primer 23fr: 5'-AAAATTAAACCCTCCTCCATCAGT-3'
2nd locus specific primer 01gf: 5'-AGACTCCAAGCGAAGAAGACTATG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11